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. 2018 Jul 18;9:2809. doi: 10.1038/s41467-018-05253-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Analysis of SpSLC9C1 expression in sea urchin sperm. a Representative western blot using protein lysates from flagella and heads of S. purpuratus sperm, from CHO cells heterologously expressing HA-tagged SpSLC9C1 (SpSLC9C1-HA), and from non-transfected CHO cells (mock). SpSLC9C1 in flagella and heads was probed with the anti-SpSLC9C1 antibody SU2 (left panel) and SpSLC9C1-HA in CHO K1 cells was probed with an HA antibody (right panel, see also Supplementary Figure 8). b Immunocytochemical analysis of sperm stained with SU2 (left) and pre-immune serum control (right). Upper panels: bright-field (DIC) microscopy. Lower panels: overlay of DIC and fluorescence images (SpSLC9C1, green; nucleus stained with DAPI, blue). Scale bars represent 10 µm