Table 3.
Regression equations, correlation coefficients (r2), standard deviation (SYX), limits of detection (LOD), limits of quantitative (LOQ), and linear ranges for 15 compounds.
| Compound | Regression equation | r2 | SYX | LOQ (μg/mL) | LOD (μg/mL) | Linear range (μg/mL) |
|---|---|---|---|---|---|---|
| 4-O-CQA (1) | y = 0.046·x – 0.045 | 0.9999 | 9.26·10−3 | 0.68 | 2.06 | 2.5–500.0 |
| 5-O-CQA (10) | y = 0.058·x – 0.043 | 0.9999 | 1.53·10−3 | 0.12 | 0.36 | 1.0–500.0 |
| 1,3-Di-O-CQA (14) | y = 0.046·x – 0.045 | 0.9999 | 6.99·10−3 | 0.51 | 1.55 | 2.5–500.0 |
| A-6-C-Glc-4″-O-Glc (19) | y = 0.029·x + 0.050 | 0.9999 | 7.37·10−3 | 0.14 | 0.42 | 1.0–500.0 |
| Q-3-O-2″RhaGlc (24) | y = 0.016·x – 0.015 | 0.9998 | 1.32·10−3 | 0.29 | 0.88 | 1.0–500.0 |
| Q-3-O-4″RhaGlc (25) | y = 0.017·x + 0.032 | 0.9998 | 4.91·10−3 | 0.51 | 1.55 | 2.5–500.0 |
| Q-3-O-6″RhaGlc (38) | y = 0.019·x – 0.026 | 0.9999 | 3.53·10−3 | 0.45 | 1.36 | 2.5–500.0 |
| Q-3-O-Glc (40) | y = 0.023·x + 0.050 | 0.9999 | 1.14·10−3 | 0.75 | 2.27 | 2.5–500.0 |
| Q-3-O-Gal (42) | y = 0.024·x – 0.016 | 0.9999 | 2.28·10−3 | 0.47 | 1.42 | 2.5–500.0 |
| 3,4-Di-O-CQA (44) | y = 0.061·x – 0.011 | 0.9999 | 2.64·10−3 | 0.79 | 2.39 | 2.5–500.0 |
| K-3-O-6″RhaGlc (45) | y = 0.022·x + 0.005 | 0.9999 | 2.08·10−3 | 0.62 | 1.88 | 2.5–500.0 |
| 3,5-Di-O-CQA (50) | y = 0.063·x – 0.046 | 0.9999 | 9.20·10−3 | 0.66 | 2.00 | 2.5–500.0 |
| Ir-3-O-Glc (54) | y = 0.034·x – 0.020 | 0.9998 | 5.29·10−3 | 0.87 | 2.64 | 2.5–500.0 |
| 4,5-Di-O-CQA (58) | y = 0.062·x – 0.026 | 0.9999 | 6.61·10−3 | 0.83 | 2.51 | 2.5–500.0 |
| Q (78) | y = 0.032·x + 0.034 | 0.9999 | 8.12·10−3 | 0.79 | 2.39 | 2.5–500.0 |
A, apigenin; CQA, caffeoylquinic acid; Glc, glucose; Ir, isorhamnetin; K, kaempferol; Q, quercetin; Rha, rhamnose; 2″RhaGlc, 2″-rhamnosyl-glycose (neohesperidose); 4″RhaGlc, 4″-rhamnosyl-glycose; 6″RhaGlc, 6″-rhamnosyl-glycose (rutinose).