Table 4.
Intra- and inter-day precision, repeatability, stability and recovery for 15 compounds.
| Compound | Precision intra-day (RSD%) | Precision inter-day (RSD%) | Repeatability (RSD%) | Stability (RSD%) | Recovery (%) |
|---|---|---|---|---|---|
| n = 5 | n = 4 | n = 7 | n = 7 | n = 5 | |
| 4-O-CQA (1) | 1.82 | 2.14 | 1.27 | 1.37 | 100.52 |
| 5-O-CQA (10) | 0.91 | 1.22 | 1.02 | 1.14 | 100.06 |
| 1,3-Di-O-CQA (14) | 1.54 | 2.39 | 1.53 | 1.17 | 101.18 |
| A-6-C-Glc-4″-O-Glc (19) | 1.62 | 1.89 | 1.78 | 1.67 | 98.15 |
| Q-3-O-2″RhaGlc (24) | 2.04 | 2.57 | 2.18 | 2.14 | 98.06 |
| Q-3-O-4″RhaGlc (25) | 2.17 | 2.94 | 2.63 | 2.10 | 98.52 |
| Q-3-O-6″RhaGlc (38) | 1.50 | 1.97 | 1.39 | 1.22 | 100.27 |
| Q-3-O-Glc (40) | 0.98 | 1.32 | 1.27 | 1.35 | 99.25 |
| Q-3-O-Gal (42) | 1.07 | 1.29 | 1.40 | 1.27 | 99.74 |
| 3,4-Di-O-CQA (44) | 1.39 | 2.04 | 1.62 | 1.29 | 99.63 |
| K-3-O-6″RhaGlc (45) | 1.25 | 1.67 | 1.35 | 1.30 | 98.07 |
| 3,5-Di-O-CQA (50) | 1.17 | 1.93 | 1.27 | 1.07 | 102.11 |
| Ir-3-O-Glc (54) | 1.67 | 2.19 | 1.92 | 1.35 | 99.32 |
| 4,5-Di-O-CQA (58) | 0.93 | 1.67 | 1.35 | 1.35 | 101.37 |
| Q (78) | 0.83 | 1.35 | 1.09 | 0.92 | 98.14 |
A, apigenin; CQA, caffeoylquinic acid; Glc, glucose; Ir, isorhamnetin; K, kaempferol; Q, quercetin; Rha, rhamnose; 2″RhaGlc, 2″-rhamnosyl-glycose (neohesperidose); 4″RhaGlc, 4″-rhamnosyl-glycose; 6″RhaGlc, 6″-rhamnosyl-glycose (rutinose).