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. 2018 Jul 18;8:10865. doi: 10.1038/s41598-018-28772-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Oxidative damage model induced by H₂O₂was established in GES-1 cells. (A) Morphological changes in GES-1 cells exposed to 4 different concentrations of H₂O₂, including 0 μM, 100 μM, 200 μM and 400 μM. (B) The effects of various H₂O₂ concentrations on cell viability in GES-1 cells, as determined by MTT assay. The cell viability was gradually decreased in a dose-dependent manner; *P < 0.05. (C) The effects of 400 μM H₂O₂ on cell viability in GES-1 cells for 0 h, 3 h, 6 h, 12 h and 24 h. The cell viability gradually declined in a time-dependent manner; *P < 0.05.