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. 2018 Jul 18;8:10886. doi: 10.1038/s41598-018-29305-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular proof of parental origin of hybrid F₁ females. Spain Q1 control and Israel Q2 control show population-specific polymorphisms: 4 bp TCTC INDEL and SNPs at positions 226 (Q1: ‘G’, Q2: ‘A’) and 232 (Q1: ‘T’, Q2: ‘G’). Unique haplotypes of the GC1 marker (Hap1–Hap12) from four F₁ females produced in reciprocal crosses between ♀Q1 × ♂Q2 (f1, f2) and ♀Q2 × ♂Q1 (f3, f4) were aligned against GC1 sequences from females produced in control crosses (Q1 control, Q2 control). The presence of both Spain Q1- and Israel Q2-specific sequences in all four females confirm that the F₁ females were hybrids between these two MED populations.