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. 2018 Jul 18;8:10886. doi: 10.1038/s41598-018-29305-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pairwise sequence divergence across the full length of mtCOI gene (1,542 bp) from whiteflies of the Africa/Middle East/Asia Minor clade. The grey horizontal line marks the commonly applied 3.5% species boundary threshold¹⁶. The Q1 consists of two sequences (Spain Q1 and Burkina Faso Q1). Grey arrows indicate the position of 5′ and 3′ barcoding regions widely used to assist with species identification across taxa.