Figure 6.

PRMT5 regulates PPARα and PGC-1α expression via AKT signaling. A, PRMT5 ^flox/flox WT or knockout MEF cells were transfected with vector or Myr-AKT and harvested 48 h post-transfection. Protein samples were analyzed by Western blotting for PPARα and PGC-1α expression. KO, knockout. B, AML12 cells were transduced with scramble or shPRMT5 viral supernatant for 24 h and then selected in medium containing 2 μg/ml puromycin for 48 h. Then cells were transfected with vector or Myr-AKT and harvested 48 h post-transfection. Protein samples were analyzed by Western blotting for PPARα and PGC-1α expression. KD, PRMT5 knockdown.