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. 2018 May 25;293(28):11230–11240. doi: 10.1074/jbc.RA118.002715

Figure 1.

Figure 1.

Comparison of YAP and TAZ knockout cells. A, schematic of the structural differences between YAP and TAZ. The domains are labeled proline-rich region (PRL), TEAD binding domain (TBD), WW domain (WW), SH3 binding domain (SH3), transcriptional activation domain (TAD), PXXOP site (PXXOP), 14-3-3 binding site (14-3-3), coiled-coil region (CC), PDZ binding domain (PDZ), and phosphodegron (PD). B, Western blots showing loss of LATS1/2, YAP, and/or TAZ. Cells were serum-starved overnight and either harvested or subsequently stimulated with serum for 60 min. Total cell lysates were used for Western blotting with the indicated antibodies. C, immunofluorescence showing YAP/TAZ (red), TEAD1–4 (green), and 4′,6-diamidino-2-phenylindole (DAPI, a DNA stain, blue) localization following either overnight serum starvation or overnight serum starvation followed by 60 min of serum stimulation. D–F, qPCR of CTGF, CYR61, and LGR5 following either overnight serum starvation (white columns) or overnight serum starvation followed by 60 min of serum stimulation (gray columns). Data are represented as ± S.D. **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001.