Figure 4.

Size exclusion chromatography of Trp^B26 hexamer. A, SEC chromatogram of insulin analogs in the presence of zinc and phenol. The void volume (V₀, black arrow) was defined by thyroglobulin (molecular mass 669 kDa). B, plot of log(molecular weight) versus elution ratio (V_e/V₀) of molecular weight standards. Linear relationship between log[MW] to elution ratio (V_e/V₀) is indicated by the red line with coefficient of determination (R²) 0.996 and parameters log[molecular weight] = −1.71 × (V_e/V₀) + 6.7012. Elution times of molecular weight standards are indicated by blue squares (labeled by molecular weight). Identity of molecular mass standards is as follows: 66 kDa, BSA; 45 kDa ovalbumin; 20 kDa, carbonic anhydrase, 17 kDa, myosin light chain; 12.4; cytochrome c, 6.5 IGF-I. Calculated M_r are given in Table 1.