Figure 5.

Pharmacology of Trp^B26 analog. A, receptor-binding affinities (isoform B). The affinity of [Trp^B26,Orn^B29]insulin was reduced by 2-fold relative to [Orn^B29]insulin (respective K_d estimates 0.14 (±0.03) and 0.07 (±0.02) nm). Color code is indicated in the inset. B, time course of [blood glucose] following i.v. injection in rats (n = 15); color code as in A. C, time course of [blood glucose] following SQ injection in the absence or presence of 0.3 mm ZnCl₂ (n = 18). D, histogram summarizing the rate of fall of [blood glucose] over the first 30 min in C, black bars indicate S.D. E, time course of [blood glucose] following SQ injection of pI-shifted analogs: [Gly^A21, Orn^B29,Orn^B31,Orn³²]insulin, and its Trp^B26 derivative (n = 6; color code in panel).