Table 2.

Thermodynamic stabilities of insulin analogs

Analog	ΔGua	Cmid	mb
	kcal mol−1	m	kcal mol−1 m−1
Wildtype	3.4 ± 0.1c	5.0 ± 0.1	0.68 ± 0.02
OrnB29	3.3 ± 0.1	4.9 ± 0.1	0.67 ± 0.01
TrpB26,OrnB29	3.3 ± 0.1	5.1 ± 0.2	0.64 ± 0.03

^a Parameters were inferred from CD-detected guanidine denaturation data by application of a two-state model; uncertainties represent fitting errors for a given data set.

^b The m-value (slope Δ(G)/Δ(M)) correlates with surface area exposed on denaturation.

^c Analysis of replicates of [Trp^B26,Orn^B29]insulin, parent [Orn^B29]insulin, and WT samples indicated that experimental standard errors were equal to or less than the above fitting errors: ±0.1 kcal mol⁻¹ (ΔG_u), ±0.1 m (C_mid), and ±0.01 kcal mol⁻¹ m⁻¹ (m).