Figure 1.

Arginine methylation of TLS inhibits the interaction with pncRNA-D. A, the pncRNA-D locus (top) and the high-affinity binding site of pncRNA-D (5′(1-1); from nucleotide 32 to 44) to TLS (bottom). B, methylation of GST-TLS by PRMT1 in vitro. The methylated TLS was detected by an anti-dimethylarginine antibody. An unrelated band is indicated with a star. C, endogenous TLS from HeLa cell nuclear extract bound to both full-length and 5′(1-1) of pncRNA-D. D, RNA pulldown assay using biotinylated pncRNA-D was performed with incubation with the unmethylated and methylated GST-TLS; 20% of the protein used for the RNA pulldown assays was loaded as input.