Table 1.

Proteins identified by mass spectrometry analysis

Part a: Striatin-1 was immunoprecipitated from striatal tissue lysate isolated from adult mice (∼6 months). Eluted proteins were identified by liquid chromatography-mass spectrometry. Score is the probability that the matched peptide is not a random event: the higher the score, the stronger the match. Expectation refers to the expectation of obtaining an equal or higher score by chance. Protein ID and Protein Name refer to the nomenclature used to identify the protein. MW is molecular weight. % coverage is the % of the protein covered by the peptide sequences. Total spectral count is a measure of the ion abundance of the species and may be used as a gross quantitative measure. Part b. Affinity purification with microcystin-Sepharose beads was performed on striatal tissue from adult rats (∼6 months). Eluted proteins were separated using SDS-PAGE and gels were stained with SimplyBlue Safe Stain (Coomassie Blue). All gel bands containing prominent proteins were cut from the gel and analyzed by mass spectrometry. Headings are the same as described in Table 1a. The expectation of 0 indicates that the identity of the protein is absolutely certain.