Table 2.

Physical parameters of the tubulin CTTs, characteristic blockage time, τ_off, obtained at −27.5 mV of applied voltage, and effective “gating charge” n of VDAC blockage calculated from the measured τ_off voltage dependences using Equation 1

τ_off and n values are expressed as a mean of 3–5 independent experiments ± S.D.

Tubulin CTT	CTT length	CTT center chargea	τoff at −27.5 mV	n
	nm		ms
βyeast	13.6	−11	322 ± 22	9.7 ± 0.1
β3	9.2	−11	227 ± 22	9.9 ± 0.3
β2	7.2	−8	4.6 ± 0.1	8.4 ± 0.2
α1	5.6	−5	1.3 ± 0.6	4.7 ± 0.1

^a CTT center charge is equal to total CTT charge excluding first five and last three amino acids.