Figure 9.

Ethanolamine-mediated rescue is specific to CL deficiency. A, growth of WT, rcf1Δ, and rcf2Δ cells in SC glucose and SC ethanol ± 2 mm Etn media was monitored at 30 °C (upper panel) and 37 °C (lower panel) by measuring absorbance at 600 nm. Data are representative of at least two independent measurements. B, 10-fold serial dilutions of WT, taz1Δ, sdh2Δ, bcs1Δ, shy1Δ, and atp12Δ cells were seeded onto the indicated media, and images were captured after 2 days of growth on SC glucose and 5 days on SC ethanol ± 2 mm Etn at 30 °C. Data are representative of at least three independent trials. C, mitochondria from YP galactose grown WT, sdh2Δ, bcs1Δ, and shy1Δ cells were subjected to Western blot analysis. Mitochondrial respiratory chain complexes II, III, and IV subunits were probed using anti-Sdh2, anti-Rip1, and anti-Cox2 antibodies, respectively. Porin was used as a loading control.