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. 2018 Jun 4;293(28):10870–10883. doi: 10.1074/jbc.RA118.004014

Table 2.

Primers used in this study

The boldface letters represent sequences that are complementary to the flanking sequences of the hygromycin and clonNat (nourseothricin) resistance cassettes of pFA6a-hphNT1 or pFA6a-natNT2 plasmids, respectively.

Name Sequence (5′ to 3′)
COX4F CCCGTTCTCGAGCAGCTGACTGTCCACCAATAAGATC
COX4R CCCGGTGGATCCCGAAGCAATACCGGTAAGCTGCTTA
TAZ1 S1 CATTTTCAAAAAAAAAAAAAGTAAAGTTTTCCCTATCAAATGCGTACGCTGCAGGTCGAC
TAZ1 S2 TGAAATTTAAGCAATTAAATTCGTGTAAATACTAGCATGTAATCGATGAATTCGAGCTCG
ECT1 S1 AATGCTTTACAGGATCGGGACTTGAAATATACTGACTGGATGCGTACGCTGCAGGTCGAC
ECT1 S2 CCATTTAATTTACGTTCGAAGAAGTTTTCAACATTTGTTTAATCGATGAATTCGAGCTCG
EKI1 S1 TAGCAGAAATTAACAGATACAGATCTGCAATTTGGCATAATGCGTACGCTGCAGGTCGAC
EKI1 S2 ATCGCAGTGAATAGAAAAATACTTGATTGTGTATACAGCTTATCGATGAATTCGAGCTCG
CKI1 S1 TACACACACATAGATACGCACGTAAAATTAGAGCAAAAGATGCGTACGCTGCAGGTCGAC
CKI1 S2 TTATTTCCTTGGCCTTTGTTGAAGGAATTCGTATACGTATTATCGATGAATTCGAGCTCG