Figure 1.

A, gene expression analysis of Cited2 by RT-qPCR in WT and Vezf1^−/− ESCs showed a 4–5-fold higher expression of Cited2 in Vezf1^−/− ESCs. Gene expression was normalized to Gapdh and represented relative to gene expression in WT ESC, set to 1. B, 50 μg of total cell extract were loaded from the WT and Vezf1^−/− ESCs followed by Western blot analysis using anti-Cited2 antibody and anti-GAPDH antibody for loading control. C, screen shot from UCSC genome browser showing the occupancy of Ser²-phosphorylated Pol II at the Cited2 locus in WT and Vezf1^−/− ESCs. These data were obtained from the previously published publically available ChIP-SEQ data. WT, wildtype ESCs; Vezf1^−/−, Vezf1-knockout ESCs.