Figure 4.

A–D, gene expression analysis in WT and Vezf1^−/− D6 ECs, differentiated using 20, 40, and 60 ng/μl of VEGF-A₁₆₅. E–H, gene expression analysis in WT and Vezf1^−/− D8 ECs differentiated using 20, 40 and 60 ng/μl of VEGF-A₁₆₅. The expression of all genes is increased in D8 compared with D6 post-differentiation. Higher doses of VEGF-A₁₆₅ have no further effect on expression of VEGF-A, Flk1, Hif-1α, and Flt1 in both WT or in Vezf1^−/− cells. The data represents average and S.D. of 3 to 4 replicates. WT, wildtype; Vezf1^−/−, Vezf1 knockout; UD, 20, 40, 60 ng/μl of VEGF-A₁₆₅ used for differentiation.