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. 2018 Jul 18;38(29):6513–6526. doi: 10.1523/JNEUROSCI.0674-18.2018

Figure 5.

Figure 5.

Newborn dentate GCs lack functional GIRK channels. A, Top, Representative holding current from a mature GC held at −70 mV (left) and input resistance measured at 30 s intervals (right). Bottom, Baclofen (Bac; 30 μm; open black symbols) increased Ih values and reduced input resistance in a manner blocked by GCP55845 (CGP; 10 μm; open red symbols). Repeated-measures ANOVA: F(2,14) = 25.55 and F(2,14) = 62.24 for standing outward current and input resistance, respectively. Tukey's multiple-comparison post hoc test: ***p < 0.001; **p < 0.01. B, Top, Representative holding current and input resistance from a newborn GC held at −70 mV, measured every 30 s. Bottom, all-points histograms made from 30 s segments show no effect of Ba2+ (500 μm, left) using an intracellular solution with γS or ML297 (10 μm, right) using GTP+ internal. C, D, Summary of holding current and input resistance changes resulting from other metabotropic agonists coupled to GIRK channels in mature GCs (solid symbols) and newborn GCs (open symbols). Baclofen, 30 μm; 5-HT, 100 μm; CPA, 50 nm; and carbachol, 50 μm. Some experiments are performed at 22°C. Statistical one-sample t test differences from sample population against a theoretical mean value of 0 in C and 100 in D. ***p < 0.001; **p < 0.01.