FIG 6.

CgMed3AB affects transcription by regulating the cellular acetyl-CoA level. (A) Cellular acetyl-CoA levels in wild-type, Cgmed3abΔ, and Cgmed3abΔ/(Cgmed3ab)_OE strains. (B) Cells were cultivated in YNB medium for 48 h, washed three times with PBS, and added to prewarmed medium containing only 10 mM sodium acetate. The graph shows Cgcln3 mRNA levels measured every 5 min by RT-PCR and normalized to act1. (C) Cgcln3 mRNA levels in the Cgacs1Δ Cgacs2-ts strain at 25°C and 37°C following repletion with 1 mM acetate (cells were collected and added to 1 mM acetate and then grown at 30°C). (D) Cgcln3 mRNA levels in the Cgicl1Δ strain. (E) Median cell sizes (femtoliters [fl]) and percentages of budding were measured in the wild type and the CAGL0M11990gΔ strain. (F) Cgcln3 mRNA levels following in vitro regulation of the cellular acetyl-CoA level by adding 5 mM, 10 mM, and 15 mM acetate. (G) Cgmed3ab mRNA levels following in vivo regulation of the cellular acetyl-CoA level. The histograms represent cellular acetyl-CoA, and the circles represent the mRNA levels of Cgcln3. All experiments were repeated three times; the error bars represent SD. *, P < 0.05; **, P < 0.01.