TABLE 1.
Characteristics of pure cultures of sludge bacteria isolated from a full-scale MBR
| Sl. no. | Isolate | Identification by 16S rRNA gene sequence (% homology) | Gram staina | Shapeb | Size (μm)c | Wt or concn from incubation for 48 h in glucose-yeast extract broth |
Protein/polysaccharide ratio | Viscosity (mPa/s) | Zeta potential (mV) | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| EPS dry wt (g/liter) | Protein (mg/liter) | Polysaccharide (mg/liter) | |||||||||
| 1 | JSB1 | Serratia sp. (100) | N | R | 1.9 by 0.7 | 1.28 ± 0.77 | 43.6 | 249 | 0.17 | 1.94 | −9.14 |
| 2 | JSB2 | Bacillus sp. (99) | P | R | 4.1 by 1.6 | 0.81 ± 0.04 | 12.4 | 111 | 0.11 | 1.96 | −5.32 |
| 3 | JSB3 | Bacillus sp. (100) | P | R | 2.2 by 0.8 | 0.83 ± 0.10 | 12.2 | 146 | 0.08 | 2.02 | −11.00 |
| 4 | JSB4 | Aeromonas sp. (100) | N | R | 2.1 by 0.8 | 1.13 ± 0.65 | 38.0 | 255 | 0.15 | 1.98 | −15.30 |
| 5 | JSB6 | Bacillus sp. (100) | P | R | 2.7 by 0.8 | 1.10 ± 0.24 | 10.2 | 192 | 0.05 | 2.37 | −13.80 |
| 6 | JSB7 | Bacillus sp. (99) | P | R | 3.6 by 1.1 | 0.87 ± 0.02 | 53.0 | 227 | 0.24 | 2.00 | −6.72 |
| 7 | JSB8 | Aeromonas sp. (100) | N | R | 1.6 by 0.6 | 0.67 ± 0.14 | 28.0 | 77 | 0.37 | 1.92 | −1.85 |
| 8 | JSB9 | Aeromonas sp. (100) | N | R | 3.0 by 0.9 | 0.58 ± 0.12 | 10.6 | 159 | 0.68 | 1.99 | −3.26 |
| 9 | JSB10 | Bacillus sp. (99) | P | R | 2.0 by 1.3 | 0.89 ± 0.11 | 30.6 | 290 | 0.1 | 1.96 | −4.23 |
| 10 | JSB12 | Aeromonas sp. (99) | N | R | 1.8 by 0.8 | 0.69 ± 0.03 | 10.2 | 199 | 0.05 | 1.98 | −1.61 |
| 11 | JSB13 | Bacillus sp. (100) | P | R | 3.1 by 1.1 | 0.86 ± 0.07 | 21.2 | 190 | 0.11 | 2.00 | −4.75 |
| 12 | JSB14 | Bacillus sp. (100) | P | R | 3.7 by 1.1 | 0.93 ± 0.22 | 34.6 | 530 | 0.06 | 1.96 | −9.10 |
| 13 | JSB15 | Klebsiella sp. (100) | N | R | 1.6 by 0.8 | 1.27 ± 0.25 | 21.4 | 269 | 0.08 | 2.08 | −9.47 |
| 14 | JSB16 | Klebsiella sp. (100) | N | R | 1.5 by 1.0 | 0.96 ± 0.43 | 14.0 | 72 | 0.2 | 2.09 | −15.90 |
| 15 | JSB17 | Bacillus sp. (100) | P | R | 2.7 by 1.1 | 0.83 ± 0.00 | 35.6 | 234 | 0.15 | 2.03 | −6.64 |
| 16 | JSB18 | Klebsiella sp. (100) | N | R | 1.1 by 0.8 | 1.12 ± 0.15 | 8.2 | 52 | 0.16 | 2.01 | −15.60 |
| 17 | JSB20 | Proteus vulgaris (99) | N | R | 1.2 by 0.7 | 0.81 ± 0.26 | 37.4 | 151 | 0.24 | 1.97 | −8.24 |
| 18 | JSB21 | Vagococcus sp. (99) | P | C | 0.8 | 0.60 ± 0.08 | 12.8 | 190 | 0.07 | 2.01 | −15.50 |
| 19 | JSB22 | Bacillus sp. (99) | P | R | 1.8 by 1.3 | 0.83 ± 0.11 | 19.2 | 194 | 0.1 | 2.02 | −12.00 |
| 20 | JSB23 | Paenochrobactrum sp. (100) | N | R | 1.8 by 0.7 | 0.64 ± 0.03 | 9.8 | 133 | 0.07 | 1.89 | −17.40 |
| 21 | JSB5 | Unidentified bacterium | N | R | 4.7 by 0.8 | 1.26 ± 0.60 | 5.2 | 281 | 0.02 | 1.99 | −1.71 |
| 22 | JSB11 | Unidentified bacterium | N | R | 1.9 by 0.8 | 0.73 ± 0.11 | 6.0 | 121 | 0.05 | 2.01 | −12.10 |
| 23 | JSB19 | Unidentified bacterium | P | C | 3.7 | 0.83 ± 0.25 | 21.8 | 494 | 0.04 | 2.02 | −17.20 |
a
N, negative; P, positive.
b
R, rod; C, coccus.
c
Single values represent the cell diameter.