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. 2018 Jul 17;84(15):e01123-18. doi: 10.1128/AEM.01123-18

FIG 2.

FIG 2

Genetic organization of the L. plantarum WCFS1 chromosomal region containing the hydroxycinnamate reductase encoding genes. (A) NCBI accession NC_004567, positions 1299251 to 1309330; arrows indicate genes. The shaded genes encode proteins putatively involved in hydroxycinnamate reduction (hcr). The locations of putative promoters and transcription terminators are also indicated. The size and direction of the transcripts revealed by reverse transcription are also shown. (B) Transcriptional analysis by RT-PCR of the L. plantarum WCFS1 genome in hydroxycinnamate reductase locus. RT-PCR amplification with primers designed to amplify internal gene regions or intergenic regions: lane 2, hcrR (primers 1328 and 1329, 628 bp); lane 3, hcrR-hcrA (1327 and 1229, 861 bp); lane 4, hcrA (1228 and 1229, 241 bp); lane 5, hcrA-hcrB (1222 and 1330, 432 bp); lane 6, hcrB (877 and 878, 758 bp); lane 7, hcrB-hcrC (1385 and 1052, 778 bp); lane 8, hcrC (1051 and 1952, 384 bp); lane 9, hcrC-lp_1427 (1226 and 1331, 323 bp); lane 10, lp_1427 (1332 and 1333, 395 bp). The control without cDNA is also shown (lane 1). Left lane, 100-bp molecular size ladder. Numbers indicate some of the molecular sizes.