Agarose gel electrophoresis of RT-PCR products generated using RNA of A. faecalis JQ135 or JQ135 ΔhpaM. Lane M, ladder DNA marker; lanes 1, 2, 3, and 4, RT-PCR products of A. faecalis JQ135 grown in MSM supplemented with 1.0 mM glycerol, 5HPA, PA, and NA, respectively; lane 5, RT-PCR products of JQ135 ΔhpaM incubated in MSM supplemented with 1.0 mM 5HPA.