TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| Alcaligenes faecalis | ||
| JQ135 | Strr; wild type; 5HPA-degrading strain; G− | 17 |
| Z10 | Strr Kmr; transposon mutant of JQ135; 5HPA growth deficient | This study |
| JQ135 ΔhpaM | Strr; hpaM deletion mutant of JQ135 | This study |
| JQ135 ΔhpaM/pBBR-hpaM | Strr Gmr; JQ135 ΔhpaM containing pBBR-hpaM | This study |
| JQ135 ΔhpaD | Strr; hpaD deletion mutant of JQ135 | This study |
| JQ135 ΔhpaX | Strr; hpaX deletion mutant of JQ135 | This study |
| JQ135 ΔhpaF | Strr; hpaF deletion mutant of JQ135 | This study |
| JQ135 ΔmaiA | Strr; maiA deletion mutant of JQ135 | This study |
| JQ135 ΔmaiA/pBBR-maiA | Strr Gmr; JQ135 ΔmaiA containing pBBR-maiA | This study |
| Sphingomonas wittichii | ||
| DC-6 | Chloroacetanilide herbicide degrader; cannot degrade 5HPA | 21 |
| DC-6/pBBR-hpaDXFM-maiA | DC-6 containing pBBR-hpaDXFM-maiA | This study |
| DC-6/pBBR-hpaDXFM | DC-6 containing pBBR-hpaDXFM | This study |
| DC-6/pBBR-maiA | DC-6 containing pBBR-maiA | This study |
| Escherichia coli | ||
| DH5α | F− recA1 endA1 thi-1 hsdR17 supE44 relA1 deoRΔ(lacZYA-argF) U169 ϕ80lacZΔM15 | TaKaRa |
| SM10λpir | Donor strain for biparental mating | 40 |
| BL21(DE3) | F− ompT hsdS(rB− mB−) gal dcm lacY1 (DE3) | Novagen |
| Plasmids | ||
| pET29a(+) | Kmr; expression plasmid | Novagen |
| pSC123 | Cmr Kmr; suicide plasmid, mariner transposon | 40 |
| pJQ200SK | Gmr; mob+ orip15A lacZα+ sacB; suicide plasmid | 41 |
| pBBR1-MCS5 | Gmr; broad-host-range cloning plasmid | 42 |
| pJQΔhpaM | Gmr; hpaM gene deletion plasmid; the upstream and downstream regions of hpaM gene fused into SacI/PstI-digested pJQ200SK | This study |
| pJQΔhpaD | Gmr; hpaD gene deletion plasmid; the upstream and downstream regions of hpaD gene fused into SacI/PstI-digested pJQ200SK | This study |
| pJQΔhpaX | Gmr; hpaX gene deletion plasmid; the upstream and downstream regions of hpaX gene fused into SacI/PstI-digested pJQ200SK | This study |
| pJQΔhpaF | Gmr; hpaF gene deletion plasmid; the upstream and downstream regions of hpaF gene fused into SacI/PstI-digested pJQ200SK | This study |
| pJQΔmaiA | Gmr; maiA gene deletion plasmid; the upstream and downstream regions of maiA gene fused into SacI/PstI-digested pJQ200SK | This study |
| pBBR-hpaM | Gmr; hpaM gene complementation plasmid; the hpaM gene fused into XhoI/HindIII-digested pBBR1-MCS5 | This study |
| pBBR-maiA | Gmr; hpaM gene complementation plasmid; the maiA gene fused into XhoI/HindIII-digested pBBR1-MCS5 | This study |
| pBBR-hpaDXFM | Gmr; hpaDXFM genes fused into XhoI/HindIII-digested pBBR1-MCS5 | This study |
| pBBR-hpaDXFM-maiA | Gmr; hpaDXFM and maiA genes fused into XhoI/HindIII-digested pBBR1-MCS5 | This study |
| pET-hpaM | Kmr; NdeI-XhoI fragment containing hpaM inserted into pET29a(+) | This study |
| pET-hpaX | Kmr; NdeI-XhoI fragment containing hpaX inserted into pET29a(+) | This study |