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. 2018 Jul 17;84(15):e02882-17. doi: 10.1128/AEM.02882-17

FIG 2.

FIG 2

The transformation efficiencies of the plasmids constructed with putative centromere regions and various lengths of the Cen2 region. (A) Schematic diagram of putative centromere and truncated versions used for identification of autonomously replicating sequences. Cen2, full-length centromere region of chromosome 2; CC2, central core region of Cen2; LOR2, left outer repeat region of Cen2; ROR2, right outer repeat region of Cen2; Cen1, full-length centromere region of chromosome 1; Cen3, full-length centromere region of chromosome 3; Cen4, full-length centromere region of chromosome 4; green, inverted repeat region; magenta, central core region. (B) Representative images of colonies formed by yeast transformants growing on selective solid medium. P. pastoris strain CBS7435 was transformed with pUC19-Cen2 (left) or pUC19-LOR2(1–111) (right) and spread on YPD solid medium containing 100 μg/ml Zeocin. (C) The number of CFU per microgram of DNA obtained by transformation of yeast cells. The standard deviation is calculated according to the number of CFU in triplicates (n = 3).