FIG 5.

Measurement of cell growth. The strain CBS7435 was grown in BMGY medium without Zeocin for 48 h. The strains P01 and CBS7435 harboring pUC19-Cen2 (Cen2), pUC19-Cen2-EGFP (Cen2-EGFP), pUC19-LOR2 (LOR2), pUC19-LOR2(1–111) [LOR2(1–111)], and pUC19-PARS1-EGFP (PARS1-EGFP) were grown in BMGY medium with 200 μg/ml Zeocin for 48 h. (A) The growth curve of yeast cells. Cell growth was automatically monitored in L-shaped test tubes by using a bio-photorecorder. (B) The final cell density (OD₆₆₀) after 48 h of cultivation. OD₆₆₀ was measured by a UV-VIS spectrophotometer. Error bars represent the standard deviations from three independent transformants (n = 3).