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Average plasmid copy number per cell. CBS7435 harboring pUC19-Cen2-EGFP (Cen2) and pUC19-PARS1-EGFP (PARS1) was grown in BMGY medium with 200 μg/ml Zeocin for 24 h. The strain T38473-EGFP (genomic integration) was grown in BMGY medium without Zeocin for 24 h. Copy number was determined by qPCR. Error bars represent the standard deviations from three independent transformants (n = 3). Statistical significance was assessed by a t test (*, P < 0.05).
