TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| BL21-CodonPlus (DE3) | F− ompT hsdS(rB− mB−) gal dcm | Agilent Technologies |
| 9511 | 3×STa-chicken ovalbumin fusion + pET28α in DH5α Kan+ | 37 |
| 9529 | e1-ovalbumin + pET28α in BL21 Kan+ | This study |
| 9546 | e2-ovalbumin + pET28α in BL21 Kan+ | This study |
| 9550 | e3-ovalbumin + pET28α in BL21 Kan+ | This study |
| 9547 | e4-ovalbumin + pET28α in BL21 Kan+ | This study |
| 9551 | e5-ovalbumin + pET28α in BL21 Kan+ | This study |
| 9552 | e6-ovalbumin + pET28α in BL21 Kan+ | This study |
| 9548 | e7-ovalbumin + pET28α in BL21 Kan+ | This study |
| 9543 | e8-ovalbumin + pET28α in BL21 Kan+ | This study |
| 9553 | e9-ovalbumin + pET28α in BL21 Kan+ | This study |
| 9544 | e10-ovalbumin + pET28α in BL21 Kan+ | This study |
| 9549 | e11-ovalbumin + pET28α in BL21 Kan+ | This study |
| 8460 | eltAB genes + pBR322 in TOP10, LT recombinant strain | 25 |
| 9646 | LT mutant with e2 substituted with MBP epitope, in DH5α Amp+ | This study |
| 9647 | LT mutant with e3 substituted with MBP epitope, in DH5α Amp+ | This study |
| 9648 | LT mutant with e4 substituted with MBP epitope, in DH5α Amp+ | This study |
| 9649 | LT mutant with e5 substituted with MBP epitope, in DH5α Amp+ | This study |
| 9650 | LT mutant with e6 substituted with MBP epitope, in DH5α Amp+ | This study |
| 9651 | LT mutant with e7 substituted with MBP epitope, in DH5α Amp+ | This study |
| 9652 | LT mutant with e8 substituted with MBP epitope, in DH5α Amp+ | This study |
| 9653 | LT mutant with e9 substituted with MBP epitope, in DH5α Amp+ | This study |
| 9654 | LT mutant with e10 substituted with MBP epitope, in DH5α Amp+ | This study |
| 9655 | LT mutant with e11 substituted with MBP epitope, in DH5α Amp+ | This study |
| Plasmids | ||
| pBR322 | Promega | |
| pMAL-c5X | NEB | |
| pET28a | Novagen |