FIG 1.

Summary of PrV pUL31 mutants. (A) The variable N termini of PrV and HSV-1 pUL31 are aligned. Basic amino acids are marked by triangles, and predicted phosphorylation sites are shown by asterisks. (B) The N-terminal amino acid sequences of the full-length, N-terminally truncated, and site-specifically mutated pUL31 are shown. Basic amino acids as well as predicted phosphorylation sites are highlighted as shown for panel A. The black bar indicates location of the predicted bipartite NLS; amino acids substituted in this study are underlined. A summary of the results is presented in the table on the right, with nuclear localization (Nuc loc) after transfection, colocalization in punctate pattern after cotransfection with pcDNA-UL34, and complementation (Comp) of PrV-ΔUL31 by stably expressing cell lines (++, wild-type; +, slightly reduced; +/−, significantly reduced; −, negative).