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In vitro replication. RK13, RK13-UL31, and cells expressing truncated pUL31 were infected with PrV-Ka or PrV-ΔUL31 at an MOI of 5 and harvested 24 h p.i. Shown are mean values from four independent experiments with the corresponding standard deviations. Statistically significant differences between the titers of infection with PrV-Ka and PrV-ΔUL31 of each transgenic cell line are indicated: ***, P < 0.001.
