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Ultrastructural analyses. RK13-UL31-N14 (A), RK13-UL31-N14-T19A (B), RK13-UL31-N14-T21A (C), and RK13-UL31-N14-T19/21A (D) cells were infected with PrV-ΔUL31 at an MOI of 1. Cells were processed for electron microscopy 14 h p.i. Bars indicate 1 μm (A and B) and 500 nM (C and D).