TABLE 1.
Primers used in this study
| Primera | Sequenceb (5′–3′) |
|---|---|
| UL31-N7 | CACA GAATTC ACA ATG CTC CTG CGG CGC |
| UL31-N11 | CACA GAATTC ACA ATG AAG TCG TCG GCC |
| UL31-N14 | CACA GAATTC ACA ATG GCC GCG CGG CGC |
| UL31-N18 | CACA GAATTC ACA ATG AAG ACG CTG ACG |
| PUL31-R | CTG AAT TCT TCG CGG CGC TCA CGG |
| S12/13A | CTC CTG CGG CGC AAG GCG GCG GCC GCG CGG CGC AAG |
| R16/17A | AAG TCG TCG GCC GCG GCG GCC AAG ACG CTG ACG CGC |
| K18A | GCC GCG CGG CGC GCG ACG CTG ACG CGC |
| T19A | GCG CGG CGC AAG GCG CTG ACG CGC GCG |
| T21A | GC AAG ACG CTG GCG CGC GCG GCC CG |
| T19/21A | GC AAG GCG CTG GCG CGC GCG GCC CG |
| A23/24R | G ACG CTG ACG CGC CGG CGC CGC GAT CGC TAC G |
a
Only one primer used for each mutagenesis reaction is shown.
b
Restriction enzyme recognition motifs are shown in italics, artificial start and stop codons are shown in boldface, and altered nucleotides are underlined.