FIG 3.

Co-IP analyses using virus-infected cell extracts demonstrate the association of host proteins with viroplasmic complexes/proteins. RNase-treated lysates from mock-infected and RRV-infected (MOI of 10) MA104 cells were prepared at 8 hpi. A total of 500 μg of the lysate was used to immunoprecipitate the cellular proteins using specific MAbs/PAbs. The immune complexes from mock-infected (C) and virus-infected (Inf) cell lysates were captured using BSA-saturated protein A-Sepharose beads and were resolved by SDS-PAGE. The lanes under 10% input (C and Inf) contain 50 μg of the lysates from control and infected cells, respectively. The viral proteins were coimmunoprecipitated using antibodies against the interacting host protein, and the viral proteins were detected by Western blotting using PAbs against NSP2, NSP5, and VP6 or MAb against VP6.