TABLE 2.
Primers for viral RNA detection and sequencing
| Reaction | Name | Strand | Sequence |
|---|---|---|---|
| RT-PCR | con-2211F | Forward | 5′-ACCCTTGTAATACCATGGATCAGC-3′ |
| DG-3393R | Reverse | 5′-CACCCGTTGAADTCYCACCARTTGGCG-3′ | |
| Second PCR | Ise-2317F | Forward | 5′-TACGTGGTTCCAATTGGGGCACC-3′ |
| Ise-3391R | Reverse | 5′-GGCGGTTAACCACTCTAAAGTTGCCCAC-3′ | |
| C7-2328F | Forward | 5′-TCCAATTGGGGCACCCAACA-3′ | |
| C7-3345R | Reverse | 5′-GCCGAACTTTCCAAGGGTAGTAAT-3′ | |
| Sequencing | Ise-2317F | Forward | 5′-TACGTGGTTCCAATTGGGGCACC-3′ |
| C7-2328F | Forward | 5′-TCCAATTGGGGCACCCAACA-3′ | |
| DG-2757F | Forward | 5′-GCHAAYTGGGAYATAGACATAAC-3′ | |
| DG-3046F | Forward | 5′-GGTTTTAYGACGGRTAYCC-3′ | |
| DG-2780R | Reverse | 5′-CCDGTTATGTCTATRTCCC-3′ | |
| DG-3046R | Reverse | 5′-GGRTAYCCGTCRTAAAACC-3′ | |
| C7-3345R | Reverse | 5′-GCCGAACTTTCCAAGGGTAGTAAT-3′ | |
| Ise-3391R | Reverse | 5′-GGCGGTTAACCACTCTAAAGTTGCCCAC-3′ |