FIG 5.

SMC5/6 is not required for optimal E1/E2-mediated DNA replication. (A) SMC6 levels in C33A cells at 4 days postransduction with the indicated shRNAs were detected by Western blotting. (B) Western blot showing SMC6 knockdown in C33A cells for the experiment whose results are shown in panel C. (C) Southern blotting using a probe for the HPV16 ori detected replicated and input DNA from C33A shGFP or shSMC6 #1 cell lines transfected with HPV16 E1 and E2 and the HPV16 ori-containing plasmid. Lane M, marker (HPV16 genome excised from the vector by digestion with the enzyme BamHI).