Specific infectivity of parental and TR-ME heterologous gO recombinants. Extracellular virions of TR, TR_MEgO, ME, ME_TRgO, ME-T, or ME-T_TRgO were analyzed by quantitative PCR for viral genomes, and PFU were determined by a plaque assay on nHDF. Average particle/PFU ratios from at least 4 independent experiments are plotted. Error bars represent standard deviations.