FIG 9.

Specific infectivity of ME virions produced under conditions of gO overexpression. nHDF or HFFFtet cells were infected with ME for 2 days and then superinfected with Ad vectors expressing either GFP or MEgO for an additional 4 days. Extracellular virions from nHDF (ME) or HFFFtet (ME-T) cells were analyzed by quantitative PCR for viral genomes, and PFU were determined by a plaque assay on nHDF. Shown are average particle/PFU ratios of virions produced in 2 independent experiments, each analyzed in triplicate. Error bars represent the standard deviations. Asterisks above fold differences indicate a P value of <0.03 (determined by Student's unpaired t test [2 tailed]).