TABLE 2.
Quantitative comparison of glycoprotein expression in TR_MEgO- and ME_TRgO-infected cellsd
| Immunoprecipitation antibodya | Extract inputb (ml) | Strain |
Fold differencef | Mean fold difference (±SD)g | |||
|---|---|---|---|---|---|---|---|
| TR_MEgO |
ME_TRgO |
||||||
| Densityc | Adjusted densitye | Density | Adjusted density | ||||
| Anti-gH | 0.04 | 68.4 | 2.3 | 29.5 | 1.0 | 2.4 | 1.6 (±0.5) |
| 0.13 | 181.6 | 6.1 | 163.1 | 5.3 | 1.1 | ||
| 0.40 | 539.5 | 18.0 | 410.6 | 13.2 | 1.4 | ||
| 1.20 | 1,697.7 | 56.6 | 1,064.0 | 34.3 | 1.6 | ||
| Anti-gL | 0.04 | ND | ND | ND | ND | ND | 1.5 (±0.4) |
| 0.13 | 196.6 | 15.1 | 153.8 | 11.8 | 1.3 | ||
| 0.40 | 645.0 | 49.6 | 508.3 | 39.1 | 1.3 | ||
| 1.20 | 2,547.5 | 196.0 | 1,269.6 | 97.7 | 2.0 | ||
| Anti-gO | 0.04 | 187.8 | 7.8 | ND | ND | ND | 19.7 (±1.7) |
| 0.13 | 945.8 | 39.4 | ND | ND | ND | ||
| 0.40 | 2,580.3 | 107.5 | 127.8 | 5.8 | 18.5 | ||
| 1.20 | 10,502.7 | 437.6 | 460.1 | 20.9 | 20.9 | ||
Seven microliters of rabbit antipeptide serum per immunoprecipitation reaction mixture.
Preparation of radiolabeled cell extracts is described in the legend to Fig. 6 and in Materials and Methods.
Pixel density of bands shown in Fig. 6B as determined using ImageJ version 1.48.
ND, band density not detected.
Density divided by the predicted number of methionine (met) and cysteine (cys) residues: TRgH (17 met, 13 cys), MEgH (17 met, 14 cys), TRgL (3 met, 10 cys), MEgL (3 met, 10 cys), TRgO (16 met, 6 cys), MEgO (18 met, 6 cys).
Adjusted density of TR_MEgO divided by adjusted density of ME_TRgO.
Average fold difference between TR_MEgO and ME_TRgO ± standard deviation.