FIG 4.

cGAS mediates the defense against replication of ΔVP22 virus. (A to D) The stably transfected HFF-shNC and HFF-shcGAS cells were infected with WT or ΔVP22 virus at an MOI of 2, and then the virus was harvested at the indicated time points postinfection and subjected to WB analysis (A and C) or viral plaque assay on Vero cells (B and D). The data represent results from one of the triplicate experiments. Statistical analysis was performed using Student's t test with GraphPad Prism 5.0 software. (* 0.01 < P < 0.05).