FIG 4.
GSCs support G207 DNA replication. (A) Quantitative PCR using HSV-1 ICP4 and cellular GAPDH primers on DNA isolated from MGG8 cells infected with G207, FΔ6, and G47Δ at an MOI of 2 at 8 and 24 hpi. Fold increase was calculated using ΔΔCT of ICP4 and GAPDH in relation to a 2-hpi time point (n = 2 to 9). There is no significant difference between replication levels of viruses in ScGC8s. *, P < 0.05 (ANOVA). (B) qPCR of GSC4s infected with G207, FΔ6, and G47Δ at an MOI of 2 for 24 h in the presence of 10 μM acyclovir (ACV; +) or vehicle (−) (n = 1). (C) Corresponding plaque assay of GSC4s infected in the presence or absence of ACV (n = 2).