FIG 8.

Phosphorylation of eIF4E does not restore TL protein translation in G207-infected GSCs. (A) Western blot for eIF4E protein from whole-cell lysates from GSC8 cells infected with G47Δ and G207 (24 hpi) at an MOI of 2 and immunoblotted with antibodies against p-eIF4E, eIF4E, gC, Us11, and vinculin (loading control). (B) Whole-cell extracts from GSC4s expressing MNK1 wild-type or the constitutively active mutant T344D and infected with G207 or G47Δ (24 hpi) at an MOI of 2, or mock (M). Immunoblotting was performed with antibodies against eIF4E, p-eIF4E, MNK1, gC, Us11, and vinculin (loading control). (C) Quantification of Western blot for MNK1 expression as fold change in MNK1/vinculin normalized to the level of mock infection with an empty vector. (D) Virus growth of G207 and G47Δ on MNK1-transduced GSC4s from the experiment shown in panel C. Values are means ± standard deviations (n = 3). G47Δ yield at 24 hpi is significantly different from that at 6 hpi (P < 0.01; ANOVA).