FIG 6.
Role of TRIM21 in antibody-mediated neutralization. (A) Measurement of in vitro neutralizing antibody titers by the L1-PBNA for mouse antisera to Cervarix or L2α11-88×5 or MAbs WW1 and RG1. The neutralization assays tested the IC50 for HPV16 infection of either wild-type mouse embryo fibroblast (MEF) cells (left) or TRIM21 knockout mouse embryo fibroblast cells (right). (B) Binding of humanized WW1 (closed circles) and humanized WW1 with TRIM21 binding-site mutations (open circles) to HPV16 L2 by an ELISA. (C) In vitro neutralization of HPV16 by humanized WW1 (closed circles) and humanized WW1 with TRIM21 binding-site mutations (open circles).