FIG 2.

SeV efficiently infects moDCs with rcSeV, eliciting higher transgene expression than rdSeV. MoDCs from 3 different HCMV seronegative blood donors were infected at the indicated MOIs, and the intracellular presence of the transgenes GFP, IE-1, and pp65 was quantified via flow cytometry after 24 (A and B) and 48 h (C and D) (nd, not determined). (A and C) Results are presented as the percentage of cells positive for a given antigen, with connected lines indicating values that were obtained using cells from an individual donor. (B and D) Median fluorescence intensity (MFI) values were normalized to the signals obtained from uninfected cells, with bars representing the means and standard deviations of values from all donors.