FIG 6.

Colocalization of UL50 proteins and UBE1L in MG132-treated cells. (A) HeLa cells in chamber slides were singly transfected with a plasmid containing the UL50-HA gene (wild type or ΔTM) or Myc-UBE1L. Forty-eight hours after transfection, double-label IFA was performed with anti-HA and anti-Myc antibodies. Hoechst dye was used to stain cell nuclei. (B) HeLa cells were cotransfected with plasmids expressing the Myc-UBE1L and UL50-HA proteins (Wt or ΔTM mutant), as indicated. Forty-eight hours after transfection, cells were left untreated or treated with MG132 (5 μM) for 16 h, and double-label IFA was performed as described for panel A. Representative confocal microscopic images are shown.