FIG 4.

Representative neuropathology and immunohistochemical assessment of gliosis and PrP deposition in cerebral cortex brain sections from control and RML-infected mice either treated or untreated with PLX5622. Mice were inoculated with either normal brain homogenate (NBH) (A to D) or scrapie strain RML (E to P) and treated with PLX5622 (A to H) or untreated (I to P). Sections of cerebral cortex were probed with antibodies that recognize PrP (A, E, I, and M), GFAP (B, F, J, and N), and Iba1 (C, G, K, and O) or stained by H&E (D, H, L, and P). Representative images of the cerebral cortex are shown for all at the scale indicated in panel A. White arrowheads in panels E, I, and M indicate patches of PrPSc deposition. Black arrows in panels H and P indicate vacuoles present in the neuropil.