FIG 4.

NS6 disrupts the IFN-β promoter activation induced by RIG-I or MDA5 coupled with SeV or poly(I·C). HEK-293T cells were transfected with IFN-β–Luc, pRL-TK, and the other indicated expression plasmids. At 24 h after transfection, cells were stimulated with SeV/poly(I·C) or were left untreated for 12 h. Dual-Luciferase assays were then performed as described in the legend to Fig. 3. The presented results represent the means and standard deviations of data from three independent experiments. ***, P < 0.001. Western blot analysis with anti-HA antibody shows the expression of NS6 protein, and Western blotting for β-actin served as a protein loading control.