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NS6 interacts with the carboxyl terminus domain of RIG-I or the helicase and carboxyl terminus domains of MDA5. HEK293T cells were cotransfected with pCAGGS-HA-NS6 and the expression plasmids encoding the 2CARD, Hel, or CTD of RIG-I/MDA5. Immunoprecipitation assays with anti-HA (A and B) or anti-Flag (C and D) antibody (IP: HA or IP: Flag, respectively) and Western blot analysis were performed as described for Fig. 5A and C.
