FIG 5 .
Production of infectious virus in HK1-EBV ALI culture. (A) Green Raji unit (GRU) titers measuring numbers of infectious units were determined for extracellular and cell-associated virus from HK1-EBV ALI cultures. Error bars were calculated from four replicate green Raji titers. Analyses of titers from week 2 were repeated in three biological replicates with the following values: 1.15 × 106 ± 3.8 × 105 GRU/ml (extracellular) and 4,000 ± 949 GRU/ml (cell associated). (B) Transmission electron micrograph (TEM) of HK1-EBV cells at week 2 of ALI culture. Herpesvirus-like particles (~250 nm) were visually identified by an independent microscopist. A packaged mature virion (arrow) and unpackaged immature virions (arrowhead) are illustrated.