FIG 4 .
Capreomycin and rifampin induce substantial changes in mycolic acid lipid components in M. smegmatis. (A) Hierarchical clustered heatmap comparing loadings obtained from cross-validated OPLS-DA of 1H HR-MAS NMR spectra of M. smegmatis mc2 155 grown under the indicated conditions. (B to F) Volcano plots are shown for individual comparisons of unchallenged bacteria and those challenged with 0.025% tyloxapol (B), D-LAK120-A (C), D-LAK120-HP13 (D), rifampin (E), or capreomycin (F). Volcano plots are of PQN normalized data and allow comparison of fold changes and significance for each metabolite. Blue, significant reductions; red, significant increases; gray, nonsignificant changes in the indicated metabolites.