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. 2018 Jul 12;9:1538. doi: 10.3389/fmicb.2018.01538

FIGURE 5.

FIGURE 5

Gene expression of IL-1β and TNF-α from bovine macrophages exposed to U. diversum and LAMPsUd and pretreated with signaling blockers for TLR4 (CLI-095), TLR2/4 (OxPAPC), and NF-κB (Celastrol). The cells were analyzed after 2 h (LAMPs) and after 12 h (U. diversum). (A) Expression of IL-1β after incubation with ATCC 49782 (105 Ureaplasma/mL) and CI-GOTA (103 Ureaplasma/mL). (B) Expression of IL-1β after incubation with LAMPsUd of ATCC 49782 and CI-GOTA (2 μg/ml). (C) Expression of TNF-α after incubation with ATCC 49782 (105 Ureaplasma/mL) and CI-GOTA (103 Ureaplasma/mL). (D) Expression of TNF-α after LAMPsUd incubation of ATCC 49782 and CI-GOTA (2 μg/ml). The treatments were compared with positive control (inoculation of U. diversum or LAMPsUd in the absence of blocker: W/B). PBS was used as negative control (CN) and 100 ng/mL of LPS and 100 ug of Pam3CysSK4 was used as positive control. Treatments were compared using the Kruskal–Wallis non-parametric test followed by the Dunn post-test. Statistically different groups are those presenting different symbols. Statistical significance (p < 0.05) is represented by the symbols (∗difference with groups with blockers, #difference with the NC group, and +difference among strains). Data are expressed as mean ± standard deviation (n = 9).